FPM

Computational High-Resolution, Wide Field of View Imaging of the Microscopic Universe.

This image illustrates our Fourier Ptychography Microscopy setup [1], which utilizes multiple green LED illuminations to capture intricate details of a butterfly antenna. By combining data from different light sources, we produce a super-resolution image that reveals the specimen’s fine structures. Unlike traditional staining methods, such as fluorescence microscopy, this technique does not alter the specimen’s properties or require staining. Instead, it images the specimen using the phase of light, allowing for the study of biological samples in their natural state.

Reconstructions

Our Reconstructions

• Leech:   https://gigapan.com/gigapans/d8fdea4c199440483a60b57a6c8a02d6
• Locus testis: https://gigapan.com/gigapans/7d65f0f06c48244dc0bc16a59e34d728
• Small intestine:  https://gigapan.com/gigapans/bf218688865ed642529c40988e34c94b

Standard FPM Reconstructions

• Leech:   https://gigapan.com/gigapans/313580a7a02556bdf3eb3ccfeca70a9b
• Locus testis: https://gigapan.com/gigapans/91a550faacc95b23880e899624695793
• Small intestine:  https://gigapan.com/gigapans/e98c74223fd53484ae8891a0a2f28393

[1] John Meshreki, Syed Muhammad Kazim, and Ivo Ihrke, “Optical system characterization in Fourier ptychographic microscopy,” Opt. Continuum 3, 2218-2231 (2024)

For further visual insights and documentation of the collaboration behind the Fourier ptychographic microscopy setup, visit the project gallery by the photographer.

Contact

Prof Dr Ivo Ihrke
Chair for Computational Sensorics and Communication Engineering
Center for Sensor Systems (ZESS)